Based on the perspective of actor network theory, this article adopts network survey, literature research, and interview methods to investigate the current situation of health information service cooperation practices of public libraries, identifies the actors involved in cooperation and their role positioning, target interests, and obstacles faced, analyzes the dynamic process of forming a public library health information service cooperation network based on the four stages of actor network translation, and then proposes optimization paths of health information services cooperation for public library, namely, seek multiparty support, and expand the health information service cooperation network; build diverse collaborative forces and form harmonious interest relationships; cocreat cooperation platform carrier, expand the channels for subject participation; improve the management evaluation system, ensure stable operation of cooperation.